FastQCFastQC Report
Plate06_barcode15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePlate06_barcode15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69130
Total Bases84.2 Mbp
Sequence length5-97208
Mean Length1218
Median Length536
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTAACACCACTGGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTT6420.93No Hit
TCTACCTCGCTAACACCACTGGTTTTCGCATTTATCGTGAAACGCTTTCG4960.72No Hit
CTAACACCACTGGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCG4070.59No Hit
ACCTCGCTAACACCACTGGTTTTCGCATTTATCGTGAAACGCTTTCGCGT3060.44No Hit
CCTCGCTAACACCACTGGTTTTCGCATTTATCGTGAAACGCTTTCGCGTT2140.31No Hit
TCGCTAACACCACTGGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTT1300.19No Hit
CAGGTCTACCTCGCTAACACCACTGGTTTTCGCATTTATCGTGAAACGCT1200.17No Hit
GCTAACACCACTGGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTC720.1No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.13.0)