FastQCFastQC Report
Plate05_barcode13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePlate05_barcode13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53383
Total Bases73.2 Mbp
Sequence length5-249771
Mean Length1371
Median Length644
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATACTCGTGTGAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTT7151.34No Hit
ACTTCCATACTCGTGTGAGTTTTCGCATTTATCGTGAAACGCTTTCGCGT2640.49No Hit
ACGACTTCCATACTCGTGTGAGTTTTCGCATTTATCGTGAAACGCTTTCG990.19No Hit
TTCCATACTCGTGTGAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTT880.16No Hit
ATACTCGTGTGAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCG770.14No Hit
CATACTCGTGTGAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTC590.11No Hit
CAGAACGACTTCCATACTCGTGTGAGTTTTCGCATTTATCGTGAAACGCT590.11No Hit
CTCGTGTGAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCGTGC560.1No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.13.0)