FastQCFastQC Report
Plate04_barcode11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePlate04_barcode11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147603
Total Bases155.9 Mbp
Sequence length5-281335
Mean Length1056
Median Length419
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAGGGAATGGAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTT24091.63No Hit
TCTATCGGAGGGAATGGAGTTTTCGCATTTATCGTGAAACGCTTTCGCGT14290.97No Hit
TTCATCTATCGGAGGGAATGGAGTTTTCGCATTTATCGTGAAACGCTTTC12580.85No Hit
GAGGGAATGGAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCGT11430.77No Hit
TCGGAGGGAATGGAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTT10930.74No Hit
CGGAGGGAATGGAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTC9240.63No Hit
GGAGGGAATGGAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCG7850.53No Hit
ATCTATCGGAGGGAATGGAGTTTTCGCATTTATCGTGAAACGCTTTCGCG5280.36No Hit
TCATCTATCGGAGGGAATGGAGTTTTCGCATTTATCGTGAAACGCTTTCG3720.25No Hit
ACCGTTTCATCTATCGGAGGGAATGGAGTTTTCGCATTTATCGTGAAACG2500.17No Hit
CTATCGGAGGGAATGGAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTT1970.13No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.13.0)