FastQCFastQC Report
Plate03_barcode09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePlate03_barcode09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172739
Total Bases178 Mbp
Sequence length5-518468
Mean Length1030
Median Length356
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGCGAGTCTTGGTTGTTTTCGCATTTATCGTGAAACGCTTTCGCGTT53963.12No Hit
CTAGGCACAGCGAGTCTTGGTTGTTTTCGCATTTATCGTGAAACGCTTTC43202.5No Hit
ACAGCGAGTCTTGGTTGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTT17160.99No Hit
TAGGCACAGCGAGTCTTGGTTGTTTTCGCATTTATCGTGAAACGCTTTCG15240.88No Hit
GCGAGTCTTGGTTGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTC9630.56No Hit
GGCACAGCGAGTCTTGGTTGTTTTCGCATTTATCGTGAAACGCTTTCGCG8520.49No Hit
CAGCGAGTCTTGGTTGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTT3920.23No Hit
GCACAGCGAGTCTTGGTTGTTTTCGCATTTATCGTGAAACGCTTTCGCGT3290.19No Hit
TTCACTAGGCACAGCGAGTCTTGGTTGTTTTCGCATTTATCGTGAAACGC2800.16No Hit
ACCAACTAGGCACAGCGAGTCTTGGTTGTTTTCGCATTTATCGTGAAACG2200.13No Hit
TTCACACAGCGAGTCTTGGTTGTTTTCGCATTTATCGTGAAACGCTTTCG2140.12No Hit
GGGTGTTTAACCAACTAGGCACAGCGAGTCTTGGTTGTTTTCGCATTTAT2020.12No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.13.0)