FastQCFastQC Report
Plate02_barcode07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePlate02_barcode07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198084
Total Bases292.7 Mbp
Sequence length5-549401
Mean Length1477
Median Length635
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGAATGAATCCTTGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTT38511.94No Hit
ACCGTGGGAATGAATCCTTGTTTTCGCATTTATCGTGAAACGCTTTCGCG30241.53No Hit
CCGTGGGAATGAATCCTTGTTTTCGCATTTATCGTGAAACGCTTTCGCGT20221.02No Hit
ACCGTGTTACCGTGGGAATGAATCCTTGTTTTCGCATTTATCGTGAAACG5110.26No Hit
CGTGGGAATGAATCCTTGTTTTCGCATTTATCGTGAAACGCTTTCGCGTT4650.23No Hit
GTTACCGTGGGAATGAATCCTTGTTTTCGCATTTATCGTGAAACGCTTTC3660.18No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.13.0)