FastQCFastQC Report
Plate01_barcode05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePlate01_barcode05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263381
Total Bases486.8 Mbp
Sequence length5-512836
Mean Length1848
Median Length933
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGGTTTGTGTAACCTTGTTTTCGCATTTATCGTGAAACGCTTTCGCG22860.87No Hit
TCCAGGGTTTGTGTAACCTTGTTTTCGCATTTATCGTGAAACGCTTTCGC15270.58No Hit
CAGGGTTTGTGTAACCTTGTTTTCGCATTTATCGTGAAACGCTTTCGCGT12610.48No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.13.0)