FastQCFastQC Report
Plate05_barcode11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePlate05_barcode11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30108
Total Bases44.9 Mbp
Sequence length5-473946
Mean Length1492
Median Length775
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAGGGAATGGAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTT1960.65No Hit
TCGGAGGGAATGGAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTT1400.46No Hit
GAGGGAATGGAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCGT1140.38No Hit
CGGAGGGAATGGAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTC980.33No Hit
TCTATCGGAGGGAATGGAGTTTTCGCATTTATCGTGAAACGCTTTCGCGT920.31No Hit
GGAGGGAATGGAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCG700.23No Hit
TTCATCTATCGGAGGGAATGGAGTTTTCGCATTTATCGTGAAACGCTTTC640.21No Hit
ATCTATCGGAGGGAATGGAGTTTTCGCATTTATCGTGAAACGCTTTCGCG480.16No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.13.0)