FastQCFastQC Report
Plate04_barcode07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePlate04_barcode07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5353
Total Bases8.3 Mbp
Sequence length5-29458
Mean Length1568
Median Length716
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGAATGAATCCTTGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTT1182.2No Hit
ACCGTGGGAATGAATCCTTGTTTTCGCATTTATCGTGAAACGCTTTCGCG641.2No Hit
CCGTGGGAATGAATCCTTGTTTTCGCATTTATCGTGAAACGCTTTCGCGT470.88No Hit
ACCGTGTTACCGTGGGAATGAATCCTTGTTTTCGCATTTATCGTGAAACG160.3No Hit
CGTGGGAATGAATCCTTGTTTTCGCATTTATCGTGAAACGCTTTCGCGTT90.17No Hit
GTTACCGTGGGAATGAATCCTTGTTTTCGCATTTATCGTGAAACGCTTTC70.13No Hit
GTGTTACCGTGGGAATGAATCCTTGTTTTCGCATTTATCGTGAAACGCTT70.13No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.13.0)