FastQCFastQC Report
Plate03_barcode19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePlate03_barcode19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683
Total Bases1.1 Mbp
Sequence length5-12198
Mean Length704
Median Length213
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTGCAGTGTCAAGAGATGTTTTCGCATTTATCGTGAAACGCTTTCGC432.55No Hit
TCGTGCAGTGTCAAGAGATGTTTTCGCATTTATCGTGAAACGCTTTCGCG261.54No Hit
GCAGTGTCAAGAGATGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTT241.43No Hit
TGCAGTGTCAAGAGATGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTT181.07No Hit
CCTCGTGCAGTGTCAAGAGATGTTTTCGCATTTATCGTGAAACGCTTTCG120.71No Hit
GTGTCAAGAGATGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCG90.53No Hit
GTGCAGTGTCAAGAGATGTTTTCGCATTTATCGTGAAACGCTTTCGCGTT70.42No Hit
GTCAAGAGATGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCGTG40.24No Hit
GTCAAGAGATGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTT40.24No Hit
CGTGCAGTGTCAAGAGATGTTTTCGCATTTATCGTGAAACGCTTTCGCGT40.24No Hit
TTCACGTGCAGTGTCAAGAGATGTTTTCGCATTTATCGTGAAACGCTTTC30.18No Hit
CCGTTCCTCGTGCAGTGTCAAGAGATGTTTTCGCATTTATCGTGAAACGC30.18No Hit
TTCACTCGTGCAGTGTCAAGAGATGTTTTCGCATTTATCGTGAAACGCTT30.18No Hit
GTGAGTGTCAAGAGATGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTT20.12No Hit
GTCAAAGAGATGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCGT20.12No Hit
GTGTCAAGAGATGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTT20.12No Hit
CCTCGTGCAGTGTCAAGAGATGTTTTCGCATTTATCGTGAAACGCTTTTC20.12No Hit
GTCAAGAGATGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCGT20.12No Hit
CTCGTGCAGTGTCAAGAGATGTTTTCGCATTTTCGTGAAACGCTTTCGCG20.12No Hit
CTCGTGCAGTGTCAAGAGATGTTTTCGCATTTATCGTGAAACGCTTTTTT20.12No Hit
GTCAAGAGATGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTT20.12No Hit
TCGTGCAGTGTCAAGAGATGTTTTCGCATTTATCGTAAACGCTTTCGCGT20.12No Hit
AGTGTCAAGAGATGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTC20.12No Hit
TCGTGCAGTGTCAAGAGATGTTTTTCGCATTTATCGTGAAACGCTTTCGC20.12No Hit
TCCTCGTGCAGTGTCAAGAGATGTTTTCGCATTTATCGTGAAACGCTTTC20.12No Hit
CTTGACACTGCACGAGGAA20.12No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.13.0)