FastQCFastQC Report
Plate02_barcode17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePlate02_barcode17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2624
Total Bases2.1 Mbp
Sequence length5-18354
Mean Length803
Median Length336
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGAAAGTACCTCTGATGTTTTCGCATTTATCGTGAAACGCTTTCGC672.55No Hit
CCAGGAAAGTACCTCTGATGTTTTCGCATTTATCGTGAAACGCTTTCGCG200.76No Hit
CTCCAGGAAAGTACCTCTGATGTTTTCGCATTTATCGTGAAACGCTTTCG190.72No Hit
CAGGAAAGTACCTCTGATGTTTTCGCATTTATCGTGAAACGCTTTCGCGT110.42No Hit
AGGAAAGTACCTCTGATGTTTTCGCATTTATCGTGAAACGCTTTCGCGTT110.42No Hit
TTCATCCAGGAAAGTACCTCTGATGTTTTCGCATTTATCGTGAAACGCTT40.15No Hit
ACCTCTGATGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCGTGC40.15No Hit
TTCACTCCAGGAAAGTACCTCTGATGTTTTCGCATTTATCGTGAAACGCT30.11No Hit
GAGGAAAGTACCTCTGATGTTTTCGCATTTATCGTGAAACGCTTTCGCGT30.11No Hit
TCAGGAAAGTACCTCTGATGTTTTCGCATTTATCGTGAAACGCTTTCGCG30.11No Hit
CCGGAAAGTACCTCTGATGTTTTCGCATTTATCGTGAAACGCTTTCGCGT30.11No Hit
CCCTCCAGGAAAGTACCTCTGATGTTTTCGCATTTATCGTGAAACGCTTT30.11No Hit
AAAGTACCTCTGATGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTT30.11No Hit
AGTACCTCTGATGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCG30.11No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.13.0)