FastQCFastQC Report
Plate01_barcode15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePlate01_barcode15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8245
Total Bases10 Mbp
Sequence length5-466635
Mean Length1220
Median Length438
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTAACACCACTGGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTT1611.95No Hit
TCTACCTCGCTAACACCACTGGTTTTCGCATTTATCGTGAAACGCTTTCG851.03No Hit
CTAACACCACTGGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCG800.97No Hit
ACCTCGCTAACACCACTGGTTTTCGCATTTATCGTGAAACGCTTTCGCGT780.95No Hit
CCTCGCTAACACCACTGGTTTTCGCATTTATCGTGAAACGCTTTCGCGTT560.68No Hit
CAGGTCTACCTCGCTAACACCACTGGTTTTCGCATTTATCGTGAAACGCT460.56No Hit
TCGCTAACACCACTGGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTT390.47No Hit
CCAGGTCTACCTCGCTAACACCACTGGTTTTCGCATTTATCGTGAAACGC300.36No Hit
ACCAGGTCTACCTCGCTAACACCACTGGTTTTCGCATTTATCGTGAAACG180.22No Hit
CTAACACCACTGGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTGG170.21No Hit
AGGTCTACCTCGCTAACACCACTGGTTTTCGCATTTATCGTGAAACGCTT160.19No Hit
GCTAACACCACTGGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTC150.18No Hit
GTCTACCTCGCTAACACCACTGGTTTTCGCATTTATCGTGAAACGCTTTC130.16No Hit
CTACCTCGCTAACACCACTGGTTTTCGCATTTATCGTGAAACGCTTTCGC110.13No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.13.0)