FastQCFastQC Report
Plate06_barcode09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePlate06_barcode09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58109
Total Bases82.3 Mbp
Sequence length5-165379
Mean Length1417
Median Length672
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGCGAGTCTTGGTTGTTTTCGCATTTATCGTGAAACGCTTTCGCGTT5240.9No Hit
CTAGGCACAGCGAGTCTTGGTTGTTTTCGCATTTATCGTGAAACGCTTTC3790.65No Hit
ACAGCGAGTCTTGGTTGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTT2700.46No Hit
TAGGCACAGCGAGTCTTGGTTGTTTTCGCATTTATCGTGAAACGCTTTCG2180.38No Hit
GCGAGTCTTGGTTGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTC1190.2No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.13.0)