FastQCFastQC Report
Plate04_barcode03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePlate04_barcode03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6222
Total Bases8 Mbp
Sequence length5-27143
Mean Length1287
Median Length445
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAGTCTTGTGTCCCAGTTACCAGGGTTTTCGCATTTATCGTGAAACGC4176.7No Hit
TTCACCGAGTCTTGTGTCCCAGTTACCAGGGTTTTCGCATTTATCGTGAA430.69No Hit
CCAGTTACCAGGGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCG160.26No Hit
CTTGTGTCCCAGTTACCAGGGTTTTCGCATTTATCGTGAAACGCTTTCGC150.24No Hit
GTGTCCCAGTTACCAGGGTTTTCGCATTTATCGTGAAACGCTTTCGCGTT110.18No Hit
CCCAGTCTTGTGTCCCAGTTACCAGGGTTTTCGCATTTATCGTGAAACGC110.18No Hit
CCGAGTCTTGTGTCCAGTTACCAGGGTTTTCGCATTTATCGTGAAACGCT100.16No Hit
TTTCACCGAGTCTTGTGTCCCAGTTACCAGGGTTTTCGCATTTATCGTGA100.16No Hit
GCCAGTCTTGTGTCCCAGTTACCAGGGTTTTCGCATTTATCGTGAAACGC80.13No Hit
GCCGAGTCTTGTGTCCCAGTTACCAGGGTTTTCGCATTTATCGTGAAACG80.13No Hit
TGTGTCCCAGTTACCAGGGTTTTCGCATTTATCGTGAAACGCTTTCGCGT80.13No Hit
GTCCCAGTTACCAGGGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTT70.11No Hit
TCCCAGTTACCAGGGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTT70.11No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.13.0)