FastQCFastQC Report
Plate03_barcode01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePlate03_barcode01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5195
Total Bases3.9 Mbp
Sequence length6-20128
Mean Length761
Median Length310
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGTCTTTGTGGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTT1132.18No Hit
TTGTCGGTGTCTTTGTGGTTTTCGCATTTATCGTGAAACGCTTTCGCGTT581.12No Hit
TCGGTGTCTTTGTGGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTT360.69No Hit
AAGAAAGTTGTCGGTGTCTTTGTGGTTTTCGCATTTATCGTGAAACGCTT210.4No Hit
ACCAAGAAAGTTGTCGGTGTCTTTGTGGTTTTCGCATTTATCGTGAAACG180.35No Hit
GTTGTCGGTGTCTTTGTGGTTTTCGCATTTATCGTGAAACGCTTTCGCGT140.27No Hit
CCAAGAAAGTTGTCGGTGTCTTTGTGGTTTTCGCATTTATCGTGAAACGC130.25No Hit
GTGTCTTTGTGGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCGT120.23No Hit
AAGTTGTCGGTGTCTTTGTGGTTTTCGCATTTATCGTGAAACGCTTTCGC110.21No Hit
GGTGTCTTTGTGGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCG100.19No Hit
AAAGTTGTCGGTGTCTTTGTGGTTTTCGCATTTATCGTGAAACGCTTTCG90.17No Hit
CGGTGTCTTTGTGGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTC60.12No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.13.0)