FastQCFastQC Report
Plate01_barcode21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePlate01_barcode21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1385
Total Bases1.1 Mbp
Sequence length5-156850
Mean Length809
Median Length139
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAGCCTCTCATTGTCCGTTCTCTAGTTTTCGCATTTATCGTGAAACGC24617.76No Hit
TTCACCGAGCCTCTCATTGTCCGTTCTCTAGTTTTCGCATTTATCGTGAA201.44No Hit
GCCTCTCATTGTCCGTTCTCTAGTTTTCGCATTTATCGTGAAACGCTTTC130.94No Hit
TCCGTTCTCTAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCGT110.79No Hit
CCGAGCCTCTCATTGTCCGTTCTCTAGTTTTTCGCATTTATCGTGAAACG90.65No Hit
CTCTCATTGTCCGTTCTCTAGTTTTCGCATTTATCGTGAAACGCTTTCGC80.58No Hit
CTCATTGTCCGTTCTCTAGTTTTCGCATTTATCGTGAAACGCTTTCGCGT70.51No Hit
CCGAGCCTCTCATTGTCCGTTCTCTTGTTTTCGCATTTATCGTGAAACGC60.43No Hit
TTTCACCGAGCCTCTCATTGTCCGTTCTCTAGTTTTCGCATTTATCGTGA60.43No Hit
CCTCTCATTGTCCGTTCTCTAGTTTTCGCATTTATCGTGAAACGCTTTCG50.36No Hit
ACCGAGCCTCTCATTGTCCGTTCTCTAGTTTTCGCATTTATCGTGAAACG40.29No Hit
CCGAGCCTCTCATTGTCCGTTCTCTAGTTTTTTCGCATTTATCGTGAAAC40.29No Hit
CCAGCCTCTCATTGTCCGTTCTCTAGTTTTCGCATTTATCGTGAAACGCT30.22No Hit
CCGAGCCTCTCATTGTCCGTTCTCTAGTTTTCGCATTTATCGTGAAACAC30.22No Hit
CCGAGCCTCTCATTGTCCGTTCTCTAGTTTTCGCATTTATCGTGGAACGC30.22No Hit
CCCGAGCCTCTCATTGTCCGTTCTCTAGTTTTCGCATTTATCGTGAAACG30.22No Hit
ATTGTCCGTTCTCTAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTT30.22No Hit
GTCCGTTCTCTAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCG30.22No Hit
TCGAGCCTCTCATTGTCCGTTCTCTAGTTTTCGCATTTATCGTGAAACGC30.22No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.22No Hit
CCCAGCCTCTCATTGTCCGTTCTCTAGTTTTCGCATTTATCGTGAAACGC30.22No Hit
CCGTTCTCTAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCGTG20.14No Hit
TTGTCCGTTCTCTAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTT20.14No Hit
CCGAGCCTCTCATTGTCCGATTCTCTAGTTTTCGCATTTATCGTGAAACG20.14No Hit
CCGAGCCTCTGATTGTCCGTTCTCTAGTTTTCGCATTTATCGTGAAACGC20.14No Hit
CATTGTCCGTTCTCTAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTT20.14No Hit
TTTAACCGAGCCTCTCATTGTCCGTTCTCTAGTTTTCGCATTTATCGTGA20.14No Hit
GTCCGTTCTCTAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCA20.14No Hit
TCCGTTCTCTAGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTT20.14No Hit
CCGACCTCTCATTGTCCGTTCTCTAGTTTTCGCATTTATCGTGAAACGCT20.14No Hit
ACGAGCCTCTCATTGTCCGTTCTCTAGTTTTCGCATTTATCGTGAAACGC20.14No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.13.0)