FastQCFastQC Report
Fri 24 Oct 2025
barcode69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebarcode69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786
Sequences flagged as poor quality0
Sequence length12-6250
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTTAATACAGTCCGAGCCTCATGTGATCTCAGCACCT90.503919372900336No Hit
AAGGTTAATACAGTCCGAGCCTCATGTGATCTCAGCACCTAAGGTTAATA70.3919372900335946No Hit
AAGGTTAATACAGTCCGAGCCTCATGTGATCTCAGCACCA50.2799552071668533No Hit
GGTGCTGAGATCACATGAGGCTCGGACTGTATTAACCTTA50.2799552071668533No Hit
GGTGCTGAGATCACATGAGGCTCGGACTGTATTAACCTTAGCAAT40.22396416573348266No Hit
GGTGCTGAGATCACATGAGGCTCGGACTGTATTAACCTTAGCAATACGT30.16797312430011196No Hit
CATATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAG20.11198208286674133No Hit
GATTTCGTCATTTTTCAAGTCGTCAAGTGGATGTTTCTCATTTTCCATGA20.11198208286674133No Hit
AGGTGCTGAGATCACATGAGGCTCGGACTGTATTAACCTTAGCAATAC20.11198208286674133No Hit
GGTGCTGAGATCACATGAGGCTCGGACTGTATTAACCTTAGCAATAC20.11198208286674133No Hit
GGTGCTGAGATCACATGAGGCTCGGACTGTATTAACCTTAGCAATA20.11198208286674133No Hit
TGAGAAACATCCACTTGACGACTTGAAAAATGACGAAATCACTAAAAAAC20.11198208286674133No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)