FastQCFastQC Report
Fri 24 Oct 2025
barcode01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebarcode01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7862
Sequences flagged as poor quality0
Sequence length9-6750
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCTAAGGTTAACA1151.462732129229204No Hit
AAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT610.7758839989824472No Hit
AAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCA390.4960569829559908No Hit
AAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCTGGTGCTGAAG340.43245993385906895No Hit
GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTA270.3434240651233783No Hit
AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTT150.1907911472907657No Hit
GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGGTGCTGAAG140.17807173747138133No Hit
AAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCAGGTGCTGAAG110.1399135080132282No Hit
AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGGTGCTGAA100.1271940981938438No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)