FastQCFastQC Report
Fri 24 Oct 2025
barcode88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebarcode88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114
Sequences flagged as poor quality0
Sequence length16-5097
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCT76.140350877192982No Hit
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCTAAGGTTAATC65.263157894736842No Hit
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCTGGTGCTGCTG32.631578947368421No Hit
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCA32.631578947368421No Hit
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCTAAGGTTAATCTTCTACTACCGATCCGAAGCAGCA21.7543859649122806No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACG21.7543859649122806No Hit
CGTCACCGTTGATGCTGGTGGCGCGCAAGCGGCGTTGTCCGTCGATCACG10.8771929824561403No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCGATCGT10.8771929824561403No Hit
GACTCCTTAGAAGTGGGAACAAAACACCCTTGGAAGGAGTTACAGAGACA10.8771929824561403No Hit
CTATATTCCCCTGTATGTGTATCGGTATTTACCAGCTGATACGGGATGGC10.8771929824561403No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTCACAATCGT10.8771929824561403No Hit
GATCAAGCCAGAAACAAGTCGTCGCGGCGGAAGTGATGGTGATACCGCGT10.8771929824561403No Hit
CGCTCGTGCCGCACAGCGTGTACGGCCTGAAACGCGACAACATCAGCTAC10.8771929824561403No Hit
GACCAAAATTATCAGAAAGATGGTGTAATGCTTTCCTATTCTTGCAGAAA10.8771929824561403No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTTAGCAATCGTA10.8771929824561403No Hit
GGGAGGAAGTACTGAAAAGTCTGACTATAGCGCATTACACCACATGCCTA10.8771929824561403No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTCACGATAAT10.8771929824561403No Hit
TCCACGGTCGGCTCAGCCACCCACGCCCACGACCCCGGCCGCGCCCGCGC10.8771929824561403No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAAGATAACCTTTAGCAATACAT10.8771929824561403No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATCG10.8771929824561403No Hit
GGCGGGGCTATGCGCACAGGCGACGCGTCTCGCGGTTGCGTATGCATTCA10.8771929824561403No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGGTGCTGCTG10.8771929824561403No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCTAAGGTTA10.8771929824561403No Hit
GTAACCTTTAAAAAAACTAAGTAAAGAAAAAACTTAATAAATGGTTTTTG10.8771929824561403No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATAAGG10.8771929824561403No Hit
GGACGTCCACCATACGTCGTTCTGGAACAGATTGACGTAGCGGTCGAAGC10.8771929824561403No Hit
GGTGCTGCTGCTTCGGGATCGGTAGTAGAAGATTAACCTACATG10.8771929824561403No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCA10.8771929824561403No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCGATGGT10.8771929824561403No Hit
AAGGTTAATCTTCTACTACGATCCGAAGCAGCAGCACCTAAGGTTAATCT10.8771929824561403No Hit
TCTCACGTGATTTGTTGCACAATACCAGGGGAACTCCCAAATACTTTGAG10.8771929824561403No Hit
GCAAGCTCTGCAGTCTCTTCCCGCAGGCCGTTGCCCTCGTTACCAATCAG10.8771929824561403No Hit
TAAGGTTAATCTTCTACTACGATCCGAAGCAGCAGCACC10.8771929824561403No Hit
GGTGCTGCTGTCTTCGGATCAGGTGGTGAAGATTAACCTTAGCAA10.8771929824561403No Hit
GCCGCGTCCGCGCACGACATCACGTGGATCATCGTCGGGCGGGTCATCCA10.8771929824561403No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACGTT10.8771929824561403No Hit
CATATAATATAAAAAATGACAATTAGTAAATGTCACATGGGAGATGAATT10.8771929824561403No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACGT10.8771929824561403No Hit
GCATAGATGTACGACAGCGTTTCTACCAATGTGGCAAAAGCAGCATCACC10.8771929824561403No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTGAAGAAATTATTAAGCGAAATATC10.8771929824561403No Hit
ACCGATCCTGCTTCTACTACCGATCCGAAGCAGCAGCACC10.8771929824561403No Hit
GCGCTGTCGCGCGTGTCGCGCGACCAGCCCACCGACAGCGGCACGCTGTT10.8771929824561403No Hit
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCAGGTGCTGCTG10.8771929824561403No Hit
GAACCGCACGCGTTCGCGTTTCCAGAAAAGCCTCGCGCGCCAGCGCGCGG10.8771929824561403No Hit
CGGCGGGCGCTGTGGCAGAGCGAGCCATCATAGCCGCGGCAGCTGCGTCT10.8771929824561403No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATCGTTTACTTCGTTCAGT10.8771929824561403No Hit
AAGGTTAATCTTCTTCTACCGATCCGAAGCAGCAGCACCTAAGGTTAATC10.8771929824561403No Hit
GCGCCAGCTCAGCGACCAGCAGGCGTTCCACGAACGTAGCGAAGCGAGCT10.8771929824561403No Hit
GGTGCTGCTGCTTCGGATCGGTAGAAATTAACCTTAGCAATA10.8771929824561403No Hit
GCCTGCGCCTCGCAGGCCTCCAGCAAGCGCGCCATCGTGAGCTGCGCGGG10.8771929824561403No Hit
GACGGCGTACCGATTCAACCTGGTCGTTCACAAAGTTAGTCATGTTCCAA10.8771929824561403No Hit
GGTGCTGCTGCTTCGGATCGGTCATCAAAGATTAACAATGTCA10.8771929824561403No Hit
CATACGCCACTCGTTATAAGCTTGCATTAACGCCTGCTCCTGCTCGCGTT10.8771929824561403No Hit
TTGTATATCATTATCCCAATTTGCTGATGGGGGATTATGATAGTTTGAGC10.8771929824561403No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACGTG10.8771929824561403No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCGATGGTTA10.8771929824561403No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACGTT10.8771929824561403No Hit
GGTGCTGCTGCTTCGGATCGGTAGTGGAAGAACCTTAGGCCATCAT10.8771929824561403No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAGCCTTTAGCAATACG10.8771929824561403No Hit
CCATTTCTGGGCCTCGATGCTGTTCTTCAACGTCGCGTTCTTCCCCATGC10.8771929824561403No Hit
ATCGAGAAAAAATTGTTATGGCCGATAGATAAATCTACTTTGACGAATAA10.8771929824561403No Hit
GGCAGATCGCCGCATGGGCGGCCGCGCCGCTGACGCGCGCGCAGCAGGAT10.8771929824561403No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACGT10.8771929824561403No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAATAA10.8771929824561403No Hit
GGTGCTGCTGCTTCGGATGAGTAGAAGATTAACCTTAGGCAATACG10.8771929824561403No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACATA10.8771929824561403No Hit
CCGTCTCTGTCCTGTTTGGCGTTGCATTTTGGCGCAAATGCGAAAGTTTT10.8771929824561403No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACTTTAGCGATACA10.8771929824561403No Hit
AGCTTCTTTTTGTGGATGGCAATCCGGCAGGCGTAAAAGCAATGTTGAAC10.8771929824561403No Hit
GTCGGGGTTGCGTCATTCGCGGGCTGATTCAGGCGCTGCAGGGCCACAAG10.8771929824561403No Hit
GCTTAAAAAGCAGCAGCGCCATCGCGAACGATACGAGACGATTTTGCCAA10.8771929824561403No Hit
CATCATGCAAATAATTACTTCAACGGATCGCAGTGAACATCTATTCCTTC10.8771929824561403No Hit
GGTGCTGCTGCTTCGT10.8771929824561403No Hit
CTGCGCCCCGGCGCCGATCCGCACGCGCACCGGCAGACGCTCGACGGCGTGACGGTCGAACCGTGAGTGCGC10.8771929824561403No Hit
ATCTGCCGCCCGCGGCCTGATTCTTCCGGAATCCTTGCATGAATGAAGCG10.8771929824561403No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATAA10.8771929824561403No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAACAGCCTAGCGATACG10.8771929824561403No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCTGAAGCAA10.8771929824561403No Hit
CTTTAATGCCTCGGATTTTTGCAAATACGCAAAAAGGAAACGCTATCAAA10.8771929824561403No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTGGTGCTGCTGC10.8771929824561403No Hit
AGGTGCTGCTGCTTCGGATCAGTAGAAGATTAACCTTAGCAAT10.8771929824561403No Hit
AGTTAATGACCAGTAAAGAAATTATGTATAGTCTGAATCTTAACCTGCCT10.8771929824561403No Hit
GTCCGTTGATTTCTTCCATAAATTCTTATTTAGTTGACAAGGTTTTCAGT10.8771929824561403No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTCACGATGGT10.8771929824561403No Hit
GCCGGTGAAGCTGACCGATAACCAGCGCGATCTGCTCAAGCAGTTCGAGC10.8771929824561403No Hit
CGCCCTTCAGGGGTAGCGAACGATAGCGAGCGTGGGGGCGGTAGCCGTTC10.8771929824561403No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCA10.8771929824561403No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTGCACGATGGTA10.8771929824561403No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTT10.8771929824561403No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCGATCGT10.8771929824561403No Hit
GCCTCAATCGGAAGGCGCTTCATTGGATATAAAGTTGAAAGAAGCAGCGA10.8771929824561403No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAG10.8771929824561403No Hit
CTACCGGATTCCGGAAGAAGTGAGAGGTCTGCATATCCTCTCCTTCCAGA10.8771929824561403No Hit
CGGTCATGCGTCCCTTACGCGGATGCCAACCGCATTTGCGCTGGCGACAC10.8771929824561403No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTGATGGT10.8771929824561403No Hit
ACTCAGACTTTGATCTAGATACACCAGCTAAACTTCGACAAGAGGCATAC10.8771929824561403No Hit
ATTTCATCATAGGTTTCAGTTGGGAATAAAACTTGTGCACATCCGTATAT10.8771929824561403No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)