FastQCFastQC Report
Fri 24 Oct 2025
barcode32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebarcode32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4493
Sequences flagged as poor quality0
Sequence length6-12522
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTTAACCAGTAGAAGTCCGACAACGTCATCAGCACCT240.5341642555085689No Hit
AAGGTTAACCAGTAGAAGTCCGACAACGTCATCAGCACCTAAGGTTAACC160.35610950367237926No Hit
AAGGTTAACCAGTAGAAGTCCGACAACGTCATCAGCACCA100.22256843979523702No Hit
AAGGTTAACCAGTAGAAGTCCGACAACGTCATCAGCACCTGGTGCTGATG90.20031159581571334No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)