FastQCFastQC Report
Thu 30 Oct 2025
barcode80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebarcode80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61
Sequences flagged as poor quality0
Sequence length39-7856
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTTAACGGATGAACATAGGATAGCGATTCCAGCACCTAAGGTTAACG1118.0327868852459No Hit
AAGGTTAACGGATGAACATAGGATAGCGATTCCAGCACCT46.557377049180328No Hit
AAGGTTAACGGATGAACATAGGATAGCGATTCCAGCACCA34.918032786885246No Hit
AAGGTTAACGGATGAACATAGGATAGCGATTCCAGCACCTGGTGCTGGAA23.278688524590164No Hit
AAGGTTAACGGATGAACATAGGATAGCGATTCCAGCACCAGGTGCTGGAA23.278688524590164No Hit
AGGTGCTGGAATCGCTATCCTATGTTCATCCGTTAACCTTTAGCAATACGTA11.639344262295082No Hit
GGGCTGGAATCGCTATCCTATGTTCATCCGTTAACCTTAGCAATAC11.639344262295082No Hit
TAAGGTTAACGGATGAACATCGAATCCTAATTACAGCACC11.639344262295082No Hit
ATGTTTATGTATTGCCCTCTACTGGTAGATTTCCGTATTGCTAGGTTAAC11.639344262295082No Hit
GGTGCTGGAATCGCTATCCTATGTTCATCCGTTAACCTTAGCGATCGTG11.639344262295082No Hit
CGTTTGCTGCGTCAGCAGCAGAGAAACGAGATTATGATCAACTTCGTTCG11.639344262295082No Hit
AAGCCAACGGATCAACATAGGATAACAATTCCCAGCACC11.639344262295082No Hit
TAAGGTTAACGGATGAACGTCAGATAGCGATTCCAGCACC11.639344262295082No Hit
AAGGTTAACGGATGAACATAGAATAGCGATTCCAGCACCAGGTGCTGGAA11.639344262295082No Hit
AGCACCTGAGGAAGTTCTTCTTTCCGGTGGCCTGGTAGTGGGCGATGGCG11.639344262295082No Hit
GCGGAACGTCAGCACGGCGGCAATGCCTCCGTCACGGGCCGCGCCTTCCA11.639344262295082No Hit
TGAGCTTAAAGCGGTGGACATAGGATAGCGATTCCAGCACC11.639344262295082No Hit
AGGTGCTGGAATCGCTATCCTATGTTCATCCGTTAACCTTAGGTGCTGGA11.639344262295082No Hit
GCGACATCGATGGACGAGGATCGCCGTGCCGCGATCGTCGATGTGACGGT11.639344262295082No Hit
TCTCTGCGGAAACAGGGCGTGATGTGCGTGGCATAAAGCGTGGTCACCAG11.639344262295082No Hit
CACCTGGTGGTTCGTAAAATTCTTGGTCGGTTTCTGACAGTTTTGGGGCGCTCTGGTGCTTATCACCATA11.639344262295082No Hit
AGGTGCTGGAATCGCTATCCTATGTTCATCCGTTAACCTTTAGCAATACGT11.639344262295082No Hit
CATATGGATTTGTAGAGGTGGGAAAGGGATGTTAGATTTACTGGGAAGGA11.639344262295082No Hit
TACGGCGTCGGCCGTTTCTCGTTCAACGTGGCCCAGGGGCGCTGCCCGAC11.639344262295082No Hit
AAGGTTAACGGATGAACATAGGATAACAATTACAGCACCTAAGGTTAACG11.639344262295082No Hit
AGGTGCTGGAATCGCTATCCTATGTTCATCCGTTAACCTTAGCAAT11.639344262295082No Hit
TCTGCGTTCGCGCGCTGTCTCCCCTGTAAAATCGGTCAAACAGCTTCTCC11.639344262295082No Hit
CATATTCTGCGCATTGTTGCGGCCGATTCCTCCCCACAGCGCGCAGCAGA11.639344262295082No Hit
TAAGGTTAACGGATGAACATAGGATAGCGAATCCAGCACCT11.639344262295082No Hit
TAAGGTTAACGGATGAACATAGGATAGCAATTCCAGCACC11.639344262295082No Hit
AGGTGCTGGAATCGCTATCCTATGTTCATCCGTTAACCTTAGCAATACGT11.639344262295082No Hit
AAGGTTAACGGATGAACATAGGATAGCGATTCCAGCACCTAGGATTGCGC11.639344262295082No Hit
GGATAAAGATTTCCAATACTAATCCACCCATTTGACAATCATGCGGAATG11.639344262295082No Hit
AAGGTTAACGGATGAACATAGGATAGCGATTGCAGCACCTAAGGTTAACG11.639344262295082No Hit
AGGTGCTGGAATCGCTATCCTATGTTCATCCGTTAACCTTAGCAATACGTTGGT11.639344262295082No Hit
TCTAACCTAATGTTGAACATAGGATAGCGATTCCAGCACC11.639344262295082No Hit
AAGGTTAACGGATGAACATCGGATAGCGATTCCAGCACCA11.639344262295082No Hit
GGTGCTGGAATCGCTATCCTATGTTCATCCGTTAACCTTAGCAATCGTG11.639344262295082No Hit
GGTGCTGGAATCGCTATCCTATGTTCATCCGTTAACCTTAGCAATACGTA11.639344262295082No Hit
AAGGTTAACGGATGAACATAGGATAGCGATTAACCTAAGGTTAACGGATG11.639344262295082No Hit
GGTGCTGGAATCGCTATCCTATGTTCATCCGTTAACCTTAGCAATATGG11.639344262295082No Hit
GGTGCTGGAATCGCTATCCTATGTTCATCCGTTAACCTTAGCAATACG11.639344262295082No Hit
AGGTGCTGGAATCGCTATCCTATGTTCATCCGTTAACCTTAGCGATACGTTGTAAC11.639344262295082No Hit
CTCGCGCAAGGCACGCCGTTCGCGTTCGAGATCAACGACGACCAGCCCGG11.639344262295082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)