FastQCFastQC Report
Fri 24 Oct 2025
barcode88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebarcode88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92
Sequences flagged as poor quality0
Sequence length24-5398
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCTAAGGTTAATC55.434782608695652No Hit
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCT55.434782608695652No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACG44.3478260869565215No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACGT22.1739130434782608No Hit
TAAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCAC22.1739130434782608No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACATCCAATGTTGAT11.0869565217391304No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTA11.0869565217391304No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGAACCTTAGCAAT11.0869565217391304No Hit
CCGCCTCGACGCCGGCGATCTCGAGTGCGACGCGCATCGGTGCGGTGAAG11.0869565217391304No Hit
GGTGTTTATGTCATCTACTCGTTCAGTTTGGTGTTGCTAAGGTTAATCTT11.0869565217391304No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACGTA11.0869565217391304No Hit
GTCGGTCAAATCGGCTACGGGCCAATTCATATAAATTCATGTCACCGAGA11.0869565217391304No Hit
ATGAGGTAGGAGCGCGCAAAATCGTGCCGACGAGGGCACGATGAAGCGAC11.0869565217391304No Hit
GCAGACTCTGCAACCGGTACGCCGGCATTCAACAAAGAGTTGAGTTTGAA11.0869565217391304No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCGATACG11.0869565217391304No Hit
TGAGTTTAACCTTCCACTACCGATCCGAAGCAGCAGCACCT11.0869565217391304No Hit
ATTTCTTCCGTAACCAGGTCACATAACATATAGGGGCGCCTGCGCCCTTC11.0869565217391304No Hit
ACAAAGGTATAACTTACCATTTCCATATCCACCCGGCATTCACTGATTTC11.0869565217391304No Hit
AAGTTGATCATCTAAACAGTCCCATATCACCTAAAGAAATAGAAGCAGTT11.0869565217391304No Hit
ATATTTATTTTGAGAAATCAGTTTTTTAATATACCTTTGTCCCAATAATT11.0869565217391304No Hit
CCGCCAGTCAATATTCTGGAGGGTGAGGTTGCGCCCGTCCTCCTCAATGC11.0869565217391304No Hit
GGTGCTGCTGCTTCGGATCGGTACTACAAGATTAACCTTAGCAACGT11.0869565217391304No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATCGTAA11.0869565217391304No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATCG11.0869565217391304No Hit
TGGCGCCATGTTGGATGATTCCATTGATAAGAAGGCGCTCCGAGAGCTTA11.0869565217391304No Hit
GGTGCTGCTGCTTCGGATCGGTAATAAAGATTAACCTTAAGCAA11.0869565217391304No Hit
TCAGGCTAATATTCTGATCCAAGCCGCAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACC11.0869565217391304No Hit
CACGAGGAGGAGTACCAGATGCTTCTGGAGCAGTTCTTCCGGATGGATGA11.0869565217391304No Hit
ATAACTTTCAGAACATATACCTGACGGTTCATCCTCCGATATTTATCGGC11.0869565217391304No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTTAGCGATCGT11.0869565217391304No Hit
TAAGGTTAATCTTCTACTATGGATCCGAAGCAGCAGCACC11.0869565217391304No Hit
AAAACTTCATTTAGAAATTAATAATTAAAAAGAATAGAACAATGAAAAAG11.0869565217391304No Hit
GGTGCTGCTGCTTCGGATAGATTAGAAGATTAAGATGCTTAGCAATGG11.0869565217391304No Hit
GGTGCTGCTGCTTCGGATCGGTAGATGAAGATTTAACCTTAAGACAATGG11.0869565217391304No Hit
GATAGATGTCGTCGGCGCTCTTGGCCTTGTCGATGCCTCCCCAGCGGAAC11.0869565217391304No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACAT11.0869565217391304No Hit
GTTACGATCGATAACCGCAAGCATCTGGAAGAGATCGTTGACGATTTGAA11.0869565217391304No Hit
GCTACAGTCTAAGGTTAACACTGCCGAAGCCTGATTAGGTGGTCTATTGGTCACAA11.0869565217391304No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATA11.0869565217391304No Hit
GCCGCTTTCCGGAAGCGAACCGCCGAATGGAATCCTTTGGAAGAGACCGA11.0869565217391304No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTCACGATGGTA11.0869565217391304No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACGA11.0869565217391304No Hit
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCAAGGTTAATCT11.0869565217391304No Hit
GTTTTCGGCCCAAAATCCGCCGTTGGCTTGCTCGAGGAATGCCGATTCTA11.0869565217391304No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAAT11.0869565217391304No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACG11.0869565217391304No Hit
TACCTCGCTGCCCTTCTACCACCCCTACGGCCTGACCTGTTCCGTCATCA11.0869565217391304No Hit
GGTGCTGCTGCTTCGGATAATGGTGGTGAAGATTAACCTTGGCACTGGTG11.0869565217391304No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAATTAACCTTAGCAAT11.0869565217391304No Hit
GGTGCTGCTGCTTCGGATCGGTGGTGACAGCCTTCACAATACAT11.0869565217391304No Hit
CGGCCGCCGGCGCGGCAGTGCCGCGCGCGCATCGCTATATCGCGGCGCTC11.0869565217391304No Hit
GGTGCTGCTGCTTCAGATGGGTCATAAAGATTAACCTTAGCAATACA11.0869565217391304No Hit
TATTTTGATCAGACATTAATTACCAAACTTTTTTCCTTTCAGGAAACAAC11.0869565217391304No Hit
CGTCGTCTTGCCCGCGCCGTTATCGCCGATCGTCGCGATGCGCTCGCCCG11.0869565217391304No Hit
CCACCCACTTCAATACCGTTGCACACCATGTCGTATGCATCTGCACGGAC11.0869565217391304No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAA11.0869565217391304No Hit
AGAAGACAAGGTAGATGTACCAGCCAGTGCGAAACCCACGGCTAAGAGCA11.0869565217391304No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAACCAATGG11.0869565217391304No Hit
CAAAACATTACTCTGGAAACATTTATCGATCGTGAAAACCATACTGGTGG11.0869565217391304No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATATGTTACATCTACTTCGTTCAG11.0869565217391304No Hit
CGATGTCGCGTGAGAAGCTGATGGAACTCGCACGCGGCCGCGAATACGAA11.0869565217391304No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGGTGCTGCT11.0869565217391304No Hit
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCTAAGGTTAATCTTCTACTACCGATCCGAAGCAGCA11.0869565217391304No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCGAT11.0869565217391304No Hit
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCAGGTGCTGCTG11.0869565217391304No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATCAGTAGG11.0869565217391304No Hit
GGTGCTGCTGCTGGTGACATGAAA11.0869565217391304No Hit
TGTGGATCGTGCCGTCGAGGATATCGTACTTCATGAAGTTGGATACCGGC11.0869565217391304No Hit
GGTGCTGCTGCTTCGGATCGGTGGTGAAACATACTTTAGATGTTG11.0869565217391304No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAACAATGGC11.0869565217391304No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTT11.0869565217391304No Hit
AAGCACGCTCACAAGAAGCACGCTTCGAAGAAGGCTGCTGCTGCCGCTGA11.0869565217391304No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAATGTTACTGTTAACCT11.0869565217391304No Hit
AGTATTACAAATCAGAGAATTGTGATTGGGCAATACATAGCCAGTAAAAA11.0869565217391304No Hit
GGGTTGGAATGTTTCAGCCGAACTGATCTTCCTCCGATTCTTAGGGAATC11.0869565217391304No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATCGTTAACCTACTTCGTTG11.0869565217391304No Hit
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCTGGTGCTGCTGCTTCGGATCGGTAGTAGAAGC11.0869565217391304No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACATA11.0869565217391304No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAAT11.0869565217391304No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)