FastQCFastQC Report
Fri 24 Oct 2025
barcode50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebarcode50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417
Sequences flagged as poor quality0
Sequence length27-3598
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCTAAGGTTAAAT13331.894484412470025No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACC348.15347721822542No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCAAGGTTAAATG235.515587529976019No Hit
GGTGCTGACGCAGGAAGTTACCAAAGTCCATTTAACCTTAAGGTTAAATG215.0359712230215825No Hit
GGTGCTGACGCAGGAAGTTACCAAAGTCCATTTAACCTTGGTGCTGACGC204.796163069544365No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCAC204.796163069544365No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCGGTGCTGACGC174.07673860911271No Hit
GGTGCTGACGCAGGAAGTTACCAAAGTCCATTTAACCTT133.117505995203837No Hit
GGTGCTGACGCAGGAAGTTACCAAAGTCCATTTAACCTTAGGTGCTGACG122.877697841726619No Hit
TAAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACC81.9184652278177456No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCTGGTGCTGACG51.1990407673860912No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCT30.7194244604316548No Hit
GGTGCTGACGCAGAAGTTACCAAAGTCCATTTAACCTT20.4796163069544364No Hit
AAGGTTAAATGGACTTTGGTAATTTCCTGCGTCAGCACCTAAGGTTAAAT20.4796163069544364No Hit
GGTGCTGACGCAGGAAGTTACCAAAGTCCATTTAACCT20.4796163069544364No Hit
GGTGCTGACGCAGGAAGTTACCAAAGTCCATTTAACCTAAGGTTAAATGG20.4796163069544364No Hit
TAAATGGACTTTGGTAACTTCCTGCGTCAGCACCAAGGTTAAATGGACTT20.4796163069544364No Hit
AGGTGCTGACGCAGGAAGTTACCAAAGTCCATTAACCTTAGGTGCTGACG10.2398081534772182No Hit
GGTGCTGACGCAGGAAGTTACCAAAGGTTAAATGGACTTTGGTAACTTCCT10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCGTGCTGACGCA10.2398081534772182No Hit
AGGTGCTGACGCAGGAAGTTACCAAAGTCCATTTAACCTTAAGGTTAAAT10.2398081534772182No Hit
GTGCTGACGCAGGAAGTTACCAAAGTCCATTTAACCTT10.2398081534772182No Hit
GGTGCTGACGCAGGAAGTTACCAAAGTCCGTTAAGGTTAAATGGACTTTG10.2398081534772182No Hit
AAGGTTAAATGGACTTGGTAACTTCCTGCGTCAGCACC10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCGGTGCTGACGCAGGAAGTTACCAATTTAACCTT10.2398081534772182No Hit
GGTGCTGACGCAGGAAGTTACCAAAGGTTAAATGGACTTTGGTAACTTCC10.2398081534772182No Hit
AAGGTTAAATGGACTTCCTGCGTCAGCACCTGGTGCTGACGCAGGAAGTT10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCTAAGGTTAAATGGACTTCCTGCGTCAGCACCA10.2398081534772182No Hit
GGTGCTGACGCAGGAAGTCCATTTAACCTT10.2398081534772182No Hit
CTGACGGAAGTGACGTTCCGGGAGCTGAAGGGAGAGGGGTGCTGACGCAG10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCAGGTGCTGACGCAG10.2398081534772182No Hit
AAGGTTAACTGGACCTTGGTAACTTTCTGCGTCAGCACC10.2398081534772182No Hit
GGTGCTGACGCAGGAAGTTACCAAAGTCCATTTAACCTTA10.2398081534772182No Hit
ACGGTGCTAACGCAGGAACCCAAAGTCCATTTAACCTT10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCGGCAA10.2398081534772182No Hit
GAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACC10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGC10.2398081534772182No Hit
TAAGGCTAAATAGTTAACTTCCTGCGTCAGCACCTAAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACC10.2398081534772182No Hit
GGTGCTGACGCAGGAAGTTACCAAAGTCCATTTAACCTTAAAGGTTAAATGGACTTTGGTAACTTCC10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCAGGTGCTGACG10.2398081534772182No Hit
GGTGCTGACGCAGGAAGTTACCATTTAACCTTAGGTGCTGACGCAGGAAG10.2398081534772182No Hit
GGTGCTGACGCAGGAAGTTACCAAAGTCCATTTAACCTTGCTGACGCAGG10.2398081534772182No Hit
GTTACCAAGTTACTTGGTTCAGTTCGTGCTAAGGTTAAATGGACTTTGGTAACTTCCTGCATAAGCACCT10.2398081534772182No Hit
GACGCAGGAAGTTACCAAAGTCCATTTAACCTTA10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCGGTGCTGACGCAGGAAGTTACCTTA10.2398081534772182No Hit
ATACGCTTCAGTTACGTAACCTTAAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACC10.2398081534772182No Hit
GGTGCTGACGCAGGAAGTTACCAAAGTCCCTTAACCTTAGGTTAAATGGA10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTGCGTCAGCACC10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCGGTGCTGACGCAGCATTTAACCTTA10.2398081534772182No Hit
CCAAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACC10.2398081534772182No Hit
GGTGCTGACGCAGGAAGTTACCAAAGTCCATTTAACCTTGTGCTGACGCA10.2398081534772182No Hit
ATGTTGTATGTTGGCGCCCGTTCAGTTACGTATTGCTAAGGTTAAATGGA10.2398081534772182No Hit
GACTTTGGTAACTTCCTGCGTCAGCACCAAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACC10.2398081534772182No Hit
GGTGCTGACGCAGGAAGTTACCAAAGT10.2398081534772182No Hit
TAAGGTTAAACAGACATGGAACTTCCTGCGTCAGCACC10.2398081534772182No Hit
GGTGCTGACGCAGGAAGTTGGCAGAAGTCCATTTAACCTTAGGTGCTGAC10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGTGCTGACGCAGGAAG10.2398081534772182No Hit
AGTATAACACAGGAAGTTACCAAAGTCCATTTAACCTAGGAT10.2398081534772182No Hit
TAAGGTTAAATGGACTTCCTGCGTCAGCACCTAAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACC10.2398081534772182No Hit
AGGTGCTGACGCAGGAAGTTACCAAAGTCCATTTAACCTTGCGGTGCTGA10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCTGGTGCTGACGCAGGAAGTTAACCTTA10.2398081534772182No Hit
GGTGCTGACGCAGGAAGTTACCAAAGTCCATTTAACCTTAGGTGCTGACGCAGAAGTCCATTTAACCTTA10.2398081534772182No Hit
ATGCCCTATTTCTTGGTTCAGATTACATCTGCTCAGCTTAGATAATGCTG10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCTCGAATCGGAA10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTACGTCAGCACCTAAGGTTAAAT10.2398081534772182No Hit
GAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCTGAGGTTAAAT10.2398081534772182No Hit
CTAAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCTAAGGTTAA10.2398081534772182No Hit
ACCTACTGGTAGGTTTACGTATTGCTAAGGTTAAATGGACTTTGGTAAGGTTCGCGTAGCACCT10.2398081534772182No Hit
GTGAAGTCTCCACCGATGATCAAGTCGAAATAAACGGGAAAGCAACCAGT10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCTAAGGTTAAATGGACTTCCTGCGTCAGCACC10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCTAAGGTTGAAT10.2398081534772182No Hit
GGTGCTGACGCAGAAGTTACCAAAGTCCATTTAACCTTAGGTGCTGACGC10.2398081534772182No Hit
GGTGCTGACGCAGGAAGTTACCAAAGTCCATTTAACCTTTTTCCTTTTCT10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCTAAGGTTAAATGGACTTTGGTAACTTCCTGCGTC10.2398081534772182No Hit
TAAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCAC10.2398081534772182No Hit
CGGTGCCGGCTTCGCTGAAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACC10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCGGTGCTGACGCAGGAAGTTACCATTTAACCTT10.2398081534772182No Hit
AACCGATAGCGGTAGGGGCTGATTCTTTCATTATCGTACGTATAATTGAC10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCAAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGC10.2398081534772182No Hit
GCTGACGCAGGAAGTTACCAAAGTCCATTTAACCTT10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCACACGGTGCTGACGCAG10.2398081534772182No Hit
AAGTTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCTAAGGTTAAAT10.2398081534772182No Hit
TCCTGACGTAAGGTGATGGATTTGGTAACTTCCTGTGGTGGTGGGGTGAT10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACA10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCCAAGGTTAAAT10.2398081534772182No Hit
GGTGCTGACGCAGAAGTTACCAAAGTCCATTTAACCTTGGTGCTGACGCA10.2398081534772182No Hit
AGGTGCTGACACAGGAAGTCTAGAAGTCATTCAGCCTTAAGTGCTGACAC10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCGGGGCTGACGC10.2398081534772182No Hit
AAGGTAATGGACTTGGGTAACTTCCTGGCATCAGCACCTAAGGTAATGGA10.2398081534772182No Hit
CAAGGTTAAATGGACTTTGGTAACTTCCTACATTTAACACCAAGGTTAAA10.2398081534772182No Hit
ACGGAAAGACGATTGTAAAGGAATGGCTGCATCAATTGCTCAGCCCTGAG10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCGGTGCTGCAGG10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCC10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCAAGGTTAAATGGACTTTGGTAACTTCCTGCGTC10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGGTGCTGACGCAGGAA10.2398081534772182No Hit
AAGGTTAAATGGATTTTGGTAACTTCCTGCGTCAGCACCTAAGGTTAAAT10.2398081534772182No Hit
TGGTGCTGACGCAGGAAGTTACCAAAGTCCATTTAACCTTAAGGTTAAAT10.2398081534772182No Hit
AGAGCAGTTTGCTGCATACTGTGGTGAGTTATTCTGAAGGTTAAATGGACTTTGGTAGCCGCGTCAGCACC10.2398081534772182No Hit
GGTGCTGACGCAGGAAGTTACCAAAGTCCGTTAACCTTAAGGTTAAATGG10.2398081534772182No Hit
GGTGCTGACGCAGGAAGTCCATTTAACCTTAAGGTTAAATGGACTTTGGT10.2398081534772182No Hit
TTTTTTAATGTCCACGCCGACCATTACGTATTGCTAAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACC10.2398081534772182No Hit
GTCTGACGCAGGAAGTTACCAAAGTCCATTTAACCTTTCATACAATTTAG10.2398081534772182No Hit
GGTGCTGACGCAGGAAGTACCAAAGTCCATTTAACCT10.2398081534772182No Hit
GACGCTGACGCAGGAAGTTACCAAAGTCCATTTAACCTTAGGTGCTGACG10.2398081534772182No Hit
AAGGTTAAATCAACTTTGGTAACTTCCTGCGTCAGCACCA10.2398081534772182No Hit
GGTGCTGACGCAGGAAGTTACCAAAGTCCATTTAACCTTAAGGTTAAATGGACTTTGGTAATTTCC10.2398081534772182No Hit
GCAGTTATCTAAGCAGAAGTTACCAAAGTCCATTTAACCT10.2398081534772182No Hit
GGTGCTGACGCAGGAAGTTACCAAAGTCCTT10.2398081534772182No Hit
GGTGCTGACGCAGGAAGTTACCAAAGCCCATTTAACCTTAAGGTTAAATG10.2398081534772182No Hit
AAGGTTAAATGGACTTCCTGCGTCAGCACCTAAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACC10.2398081534772182No Hit
CAGGTTAAATGGACTTGGTAACTTCCTGCGTCAGCACCGGTGCTGACGCA10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCAGTGCTGACGC10.2398081534772182No Hit
GGTGCTGACGCAGGAAGTTACCAAAGTCCATTTAACCTTAGGTGCTGACGCAGGAAGTTACCAAAGTCC10.2398081534772182No Hit
GGTGCTGACGCAGAAGTTACCAAAGTCCATTTAACCTTAAGGTTAAATGG10.2398081534772182No Hit
AAGGTTAAATGGACTTTGGTAACTTCCTGCGTCAGCACCA10.2398081534772182No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)