FastQCFastQC Report
Fri 24 Oct 2025
barcode20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebarcode20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365
Sequences flagged as poor quality0
Sequence length33-1198
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCACCTAAGGTTAATT21759.45205479452055No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCACCGGTGCTGATGA184.931506849315069No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCACC174.657534246575342No Hit
GGTGCTGATGAGTTCTCGTAACAGGACGCAATTAACCTTAGGTGCTGATG154.10958904109589No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCACCTGGTGCTGATG102.73972602739726No Hit
GGTGCTGATGAGTTCTCGTAACAGGACGCAATTAACCTT102.73972602739726No Hit
AGGTGCTGATGAGTTCTCGTAACAGGACGCAATTAACCTTAGGTGCTGAT71.9178082191780823No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCACCT71.9178082191780823No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCACCAGGTGCTGATG30.821917808219178No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCACCAAGGTTAATTG20.547945205479452No Hit
GGTGCTGATGAGTTCTCGTAACAGGACGCAATTAACCTTGGTGCTGATGA20.547945205479452No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCACCAGTAAGAGTTCTCGTAACAGGACGCAATTAACCTTA10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCACCTAAGGTTAATTGCGTCCTGTTACGAGAACTCA10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCACCGGTGCTGATGAGTTCTCGCAATTAACCTTA10.273972602739726No Hit
AAGGTTAATTAACTTCTCAGAACTCATCAGCACCGGTGCTGATGAGTTCC10.273972602739726No Hit
AAGGACTCATGCTCAGAACTCATCAGCACCTAAGCTCATTGCGTCCTGTT10.273972602739726No Hit
AAGGTTCATTGCGTCCTGTTACGAGAACTCATCAGCACCTGGTGCTGATG10.273972602739726No Hit
AAGGTTAATTTGGTTCCTGTTACAAGAACTCGTGGGCACCTAAGGTTGAA10.273972602739726No Hit
GGTGCTGATGGATCCTCATAACGGGACGCATCAGCACTTAGGTGCTGATGAGTTCTCGTAACAGGACG10.273972602739726No Hit
GGTGCTGATGAGTTCTCGTAACAGGACGCAATTAACCTTAGTGCGTT10.273972602739726No Hit
AAGGTTAAATACGTCCTGGGGACTCATAAGCACCTAAGGTTAAATACGTC10.273972602739726No Hit
AAGTTAATTACGTCCTGTTACGAGAACTCATCAGCACCGGTGCTGATGAGTTCTCGTAACAGGACGCTTA10.273972602739726No Hit
AAGGTTAATTGCATCCTGTTACGAGAACTCATCAGCACCTAAGGTTAAAT10.273972602739726No Hit
AGGTGTTGATGAGTTCTCGTAACAGGACGCAATTAACCTT10.273972602739726No Hit
AAGGTTGATTGCGTCCTGTTACGAGAACTCATCAGCACCTAAGGTTGATT10.273972602739726No Hit
AAGGTTAATTGCGTTCTGTTACGAGAACTCATCAGCACCGGTGCTGATGA10.273972602739726No Hit
AATAATGAGTTCTCGTAACAGGACGCAATTAACCTTA10.273972602739726No Hit
CTAAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCAC10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTACGAAACTCATCACCTAAGGTTAATTGCGT10.273972602739726No Hit
AAGGTTAATTGCATCCTGTTACGAGAACTCATCAGCACCTAAGGTTAATT10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCACT10.273972602739726No Hit
AATTGCGTCCTGTTACGAGAACTCATCAGCACCTGGTGCCTCATGATTCG10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCAC10.273972602739726No Hit
AGGTGCTGATGAGTTCTCTCGTAACAGGACGCAATTAACCTTAGGTGCTG10.273972602739726No Hit
CGCAGTCCGTCCTGTTACGAGAACTCATCAGCACCTGGCGGCCGTCCGTC10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCACCTAAGGTTAAGT10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTACAAGAACTCATCAGCACCTAAGGTTAATT10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTACAAGAACTCATCAGCACCGGTGCTGATCA10.273972602739726No Hit
GGTGCTGATGAGTTCTCGTAACAGGACGCAATTAACCTTA10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCCACCGGTGCTGATG10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTTCATCAGCACCTAAGGTTAAT10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTATCAGCACCTAAGGTTAATTG10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTCGCTGCGCACGGTACTAATAA10.273972602739726No Hit
AAGGTTAGTGATGCGTCTTGTTACGAGAACTCATCAGCACCTGGTGCTGA10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTCCAGAATTCATGAGCGCCTCAAGGTTAATT10.273972602739726No Hit
AAGGTTAAGTGCGTCCTGTTACGAGAACTCATCAGCACCTAAGGTTAATT10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTACGGGAACTCATCAGCACCTAAGGTTAATT10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTCGGCACCGGTGCTGATGAGTT10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCACCTAAGGTTGCGT10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTACGAAACTCATCAGCACCTAAGGTTAATTG10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCACCTTAGCAAACAC10.273972602739726No Hit
AAGGTTGATTGCGTCCTGTTACGAGAACTCATCAGCACCTAAGGTTAATT10.273972602739726No Hit
AAGGTTATTGCGTCCTGTTACGAGAACTCATCAGCACCTAAGGTTAATTG10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCACCTAAGGTTGATT10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCAACCTAAGGTTAAT10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTAGCAGAACTCATCAGCACCTAAGGTTAATTG10.273972602739726No Hit
GGTGCTGGAACTGGTCTCGTAACAGGACGCAATTAACCTT10.273972602739726No Hit
AAGGTTTGAGGTATTGGTCCTGTTACGAGAACTCATCAGCACC10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCACTGGTGCTGATGA10.273972602739726No Hit
AGGTGCTGATCGGTTCTGATAACGGACGCAATTGACCTT10.273972602739726No Hit
TAAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCACC10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTCTCAGCACCTAAGGTTAATTG10.273972602739726No Hit
AGGTGCTGATGAGTTCTCGTAACAATTAACCTT10.273972602739726No Hit
GGTGCTGATGAGTTCTCGTAACAGGACGCAATTAACCTTCGGTGCTGATG10.273972602739726No Hit
CTCGTTCAGTTACGTATTGCTAAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCACC10.273972602739726No Hit
AAGGTTCATTGCGTCCTGTTACGAGAACTCATCAGCACCTAAGGTTAATT10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCACCTAAGGTTAAAC10.273972602739726No Hit
AAGGTTAATTGCGTCCTGTTACGAGAACTCATCAGCACCTAAGGTTAATTGCGTCCTGTTACGAGAACTCATAA10.273972602739726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)