FastQCFastQC Report
Fri 24 Oct 2025
barcode04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebarcode04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2234
Sequences flagged as poor quality0
Sequence length10-7723
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTTAATAGGGAAACACGATAGAATCCGAACAGCACCTAAGGTTAATA138962.17547000895255No Hit
GGTGCTGTTCGGATTCTATCGTGTTTCCCTATTAACCTTAGGTGCTGTTC954.2524619516562225No Hit
AAGGTTAATAGGGAAACACGATAGAATCCGAACAGCACCTGGTGCTGTTC813.6257833482542523No Hit
AAGGTTAATAGGGAAACACGATAGAATCCGAACAGCACCT793.5362578334825425No Hit
AAGGTTAATAGGGAAACACGATAGAATCCGAACAGCACC642.864816472694718No Hit
AAGGTTAATAGGGAAACACGATAGAATCCGAACAGCACCA522.3276633840644583No Hit
AAGGTTAATAGGGAAACACGATAGAATCCGAACAGCACCGGTGCTGTTCG502.2381378692927485No Hit
AGGTGCTGTTCGGATTCTATCGTGTTTCCCTATTAACCTTAGGTGCTGTT371.656222023276634No Hit
AAGGTTAATAGGGAAACACGATAGAATCCGAACAGCACCAGGTGCTGTTC281.2533572068039391No Hit
AAGGTTAATAGGGAAACACGATAGAATCCGAACAGCACCAAGGTTAATAG251.1190689346463742No Hit
GGTGCTGTTCGGATTCTATCGTGTTTCCCTATTAACCTT210.9400179051029544No Hit
GGTGCTGTTCGGATTCTATCGTGTTTCCCTATTAACCTTAAGGTTAATAG130.5819158460161146No Hit
AGGTGCTGTTCGGATTCTATCGTGTTTCCCTATTAACCTT90.40286481647269473No Hit
AAGGTTAATAGGGAAACACGATAAATCCGAACAGCACCTAAGGTTAATAG90.40286481647269473No Hit
GGTGCTGTTCGGATTCTATCGTGTTTCCCTATTAACCTTA70.3133393017009848No Hit
AAGGTTAATAGGAAACACGATAGAATCCGAACAGCACCTAAGGTTAATAG50.22381378692927484No Hit
TAAGGTTAATAGGGAAACACGATAGAATCCGAACAGCACC40.17905102954341987No Hit
AAGGTTAATAGGGAAACACGATAGAATCCGAACAGCAC40.17905102954341987No Hit
GGTGCTGTTCGGATTCTATCGTGTTTCCCTATTAACCTTGGTGCTGTTCG40.17905102954341987No Hit
AAGGTTAATAGGGAAACACGATAGAATCCGAACAGCACTTGGTGCTGTTC30.13428827215756492No Hit
AAGGTTAATAGGGAAACACGATAGAATCCGAACAGCA30.13428827215756492No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)