FastQCFastQC Report
Fri 24 Oct 2025
barcode88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebarcode88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length37-13043
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTCGCCCGTCCGGGGCAGCTCGCCGCTCAAACGTCCTTTGACCGGAAT12.4390243902439024No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTA12.4390243902439024No Hit
GTTTCTTTGGAAGGTACATTTGCCGCTTATCCCAAGAAAGTGGTGGACGA12.4390243902439024No Hit
AAGCCTGAAGGCATGAGGGTCGTATTTTATCCGACCGACGATGAAAGCAA12.4390243902439024No Hit
GCATTGATCGGACGAACTACTTCGTTGATGAATGCAGGATCGTTGTTTTC12.4390243902439024No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTTAACCTTAACAATGA12.4390243902439024No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGGTGCTGTTGCTTCGGATCGGTAGTCAAT12.4390243902439024No Hit
GCCTAGTTCAATCACAACATTCAAATTACGCCCTTTTTCAATCATCTCAT12.4390243902439024No Hit
CTTGTAGATTGCTTTTGTTAAAATGGCCATTATTGCTATGTTAATCCTAC12.4390243902439024No Hit
CCGTAAAGCAGCGTCGGTGCTTAGAGACCGTATTCTGGTCTCTTATGCAT12.4390243902439024No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACATT12.4390243902439024No Hit
GAGAAGTTAGAGAAAAAGTATGAGCTACACTTTTATCTAAATGGAAAGAT12.4390243902439024No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACGT12.4390243902439024No Hit
ATAATGAAAGAATGAAAATCGTAAAATATTTTCATGATGCTTGTTCTTGT12.4390243902439024No Hit
GCCAGTATCTCGCGCAGCTCGGGATCAAGGTCGCGTTCACGCCGTTCGGC12.4390243902439024No Hit
TACCTGTTTTCCAGCCGCCGGTTTTTCCGGTGTTTTCCGAAATTTTTCGA12.4390243902439024No Hit
CTCAGTCTATCTATACAAGGTTACCAAGCATGTCACCGTAACCAAGCGTC12.4390243902439024No Hit
AATGATACCAAGAAAAACGGAGGCCGTGTGATTTCCGTCGGAACCACAAG12.4390243902439024No Hit
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCA12.4390243902439024No Hit
GGTATAGCATTCCGGTAGAGGAAAGTGTAGAAGTAAGTCTGGATTATTTG12.4390243902439024No Hit
GGAAACCTTCATATCTGTATATGAACATCTGGATTCCTTCGACGGCAAGA12.4390243902439024No Hit
TAGTCATCTCGATCATGACGCTTGTGACCAACAAGGTGTTCAAAAGCAAG12.4390243902439024No Hit
TCACAAGTAAGCAGATGACAGAGGTAACCGACATCGATGGTCATAAAATC12.4390243902439024No Hit
GTAGTCCACCAAGCAAACGCCGTCGCCTGCCTCCAGTCCCACACTGGTAG12.4390243902439024No Hit
GAAGACCTATCCTGGTTGGATGCTTCTGAGGCCGTAACCCACAGCCGTGA12.4390243902439024No Hit
CTTTAGGCCGCTTCTGGGGTGCGCTGTTCTTTATTTTTATGACCTTTGCC12.4390243902439024No Hit
GGTGCTGCTGCTTCGGTAGTAGAAGATTAACCTTAGCAATCGTAAAAA12.4390243902439024No Hit
TATGGCCATGTATTTGGCTTGCAGTGTCTCCTGCTGTGCATGAGCAGATT12.4390243902439024No Hit
TACGCCCCACACGGAACTGCTTCTCGGCTACCTTTGGCATACCGGGAACG12.4390243902439024No Hit
TGTATTCCTGAAGCATGAGGCGTGTGATAGATTTTGGCTTTCCGAGTTGA12.4390243902439024No Hit
AGGTGACAAAGGATATATGCAGACTCCCTGCACTTCACCATGGCGTACCG12.4390243902439024No Hit
TGTTGCTCGGCCTCTAACACCTCTTGTCGCACTTCCAGAAACTTGGGGTT12.4390243902439024No Hit
AAGGTTAAAGGTCTACCTCGCTAACACCACTGCAGCACCTTGCCACCCAG12.4390243902439024No Hit
TAAGAAAGTTATGATCGAAGTTGTAGAATCTACTTTGCAAAAGGCTGCGG12.4390243902439024No Hit
TCCCCTTCAATATGTATCAGTCAGGATCACCAGGGGATGGCATCAGGCAT12.4390243902439024No Hit
GCTATGGATCGTCGCCTTGGTGGGCCGTTACCCCACCAACAAGCTAATCC12.4390243902439024No Hit
CTAAAAGGTCGAAAACATTACATTTATGTGTAACTACTTGCCTTTATCAA12.4390243902439024No Hit
ACTACGACGCCGCCCTCGCCGCACTCAAGTAATTCCCTGCTGCCGATGAG12.4390243902439024No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAAT12.4390243902439024No Hit
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCTAAGGTTAATC12.4390243902439024No Hit
TACTCCGTCCTTACCGATGGTCAGGTTGAAATTATAACTCTTTCCTGCTT12.4390243902439024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)