FastQCFastQC Report
Thu 30 Oct 2025
barcode88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebarcode88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length40-1038
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACA16.25No Hit
GATCTGGATACGGTTATGCCGGGTCTTTCCGTAAATGATTTCGGAGATTC16.25No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATGG16.25No Hit
GGCTGAGTATCATCTTTTTCTCGTTTTCCTCAAACTCACCCGAATCATAC16.25No Hit
TGATAGAACTTCAACAGTATCTACTGATGTTGATGCTGTTAAGCTATTAG16.25No Hit
TCAGCCGCATCAAAAATATCAACAATGGCTTTAAAATTTCCAGCCCATGT16.25No Hit
ACTCTCCTGGAGTGCTTTTTGCCGTTAAACAGGCTAAGTATGATAAAAAC16.25No Hit
CTGCCATCTTATACCCAAACATCGTCACGATACAAGTCGCACAATCCGAT16.25No Hit
GCCCAATGCGTTAAAAAGGACTTATGGCCAAGGACGATGTCAGCCCGATG16.25No Hit
ATTCTCTGCCGACGGTCATCCTATCGGCGATTATTTCGATGCAATGTATC16.25No Hit
AAGTGCATCTCTGGTTGCATGGTCAAGCAAAAGACGGTTTTGCTCCACGA16.25No Hit
GAAGATGATAGATCAGGATCCCCAATATCCTTCTCTGGCCTCTGTATACC16.25No Hit
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCT16.25No Hit
GCCGGAGAAGGAACCCTCATCCCAAAGGAGCCGGTTCCTTCCCTCCTCTC16.25No Hit
GGTGCTGCTGCTTCGGTAGTAGAAGATTAACCTTAGCAATACGT16.25No Hit
GGTGCTGCTGCTTCGGAACGGTAGTAGAAGATTAACCTTAGCAAGATCGT16.25No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)