FastQCFastQC Report
Sat 1 Nov 2025
barcode88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebarcode88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117
Sequences flagged as poor quality0
Sequence length7-4936
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCTAAGGTTAATC75.982905982905983No Hit
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCT75.982905982905983No Hit
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCA54.273504273504273No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACG54.273504273504273No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACGT43.418803418803419No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTA32.564102564102564No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAAT21.7094017094017095No Hit
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCTGGTGCTGCTG21.7094017094017095No Hit
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCAGGTGCTGCTG21.7094017094017095No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTTAAGACAAT10.8547008547008548No Hit
GAGAACCAATTCAATCGGGCGCTTCAAGATGTGGAAAATGAATCGCGTCC10.8547008547008548No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAAGACGATGAT10.8547008547008548No Hit
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCGGTGCTGCTGC10.8547008547008548No Hit
ATGTGACCCGACACGTGTTGCAAGTCCATGCTACCGATCCGAAGCAGCAGCACC10.8547008547008548No Hit
GGTGCTGCTGCTTCGGAATCGGTAGTAAAGATTAACCTAAGCAATATGGTTTTG10.8547008547008548No Hit
AGGTGCTGCTGCTTGCGATGGTAGTAGAAGATTAACCTTTAGGCAA10.8547008547008548No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACGTG10.8547008547008548No Hit
ACCTTGGGGTGGGCGCCTTCGAATTGAACGGCACGATCACGATCACGTCC10.8547008547008548No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCGATACGT10.8547008547008548No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACAACAATAA10.8547008547008548No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAAGCGAAAATC10.8547008547008548No Hit
TTTGACTTACTTGGTTAGATTTAGGTCTTGCTACATTAACTTCTACTACCGATCCGAAGCAGCAGCACCT10.8547008547008548No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGTTAACCTTCAGCGATA10.8547008547008548No Hit
TACTATGGTCGGATAAAAAATATCTTTCATCACTTCCAGAATCTGAATAA10.8547008547008548No Hit
TGCGCCAACCAAGTACGAATAGTTGTAGCAGAATAACCCGTAACCGCACC10.8547008547008548No Hit
CCATTCAGGTTTTCAATGAGATTTGTGGTATAGATTATTTTTCTGATTTC10.8547008547008548No Hit
TTGGTTGACATCCACCCTGTCTGTTCTCTGGATTTCAGCGGCAACCGGCT10.8547008547008548No Hit
GGTGCTGCTGCTCAGATGGTAGTAGAGATTAACCTTACAGCTTGGTGG10.8547008547008548No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCGAT10.8547008547008548No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGGTGCTGGC10.8547008547008548No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATTTT10.8547008547008548No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATAATA10.8547008547008548No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGGTGCTGCTG10.8547008547008548No Hit
GCTGCTGCTTAAATAAAGGTAGTAGAAGATTAACCTTTAGCAATCATA10.8547008547008548No Hit
AAGGTTGATCTTACTCAATCCGAAGCAGCAGCACCA10.8547008547008548No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAACAATGG10.8547008547008548No Hit
CTTAGGGTGGGCGAGGGTGGAAGAGGAATACACTTTAGAATAGGAATACA10.8547008547008548No Hit
AAGGTTAATCTTCTACTATCGATCCGAAGCAGCAGCACCTAAGGTTAATC10.8547008547008548No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAA10.8547008547008548No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATA10.8547008547008548No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCCTTAGCAATTTTTT10.8547008547008548No Hit
TAAGGTTAATCTTCTACTACAATACGAAGCAGCGGCACC10.8547008547008548No Hit
AGGTGCTGCTGCTTCGGATCAGTAGAAGATTAACCTTAGCAATACG10.8547008547008548No Hit
CCTCGGGGATATGTTGCTGACCGGGCGTTATTCCTTTAATAGCAGGAGAA10.8547008547008548No Hit
GGACAGGATGAATCCACCGAATTCATTCATACGTAACTCATTAGCTAATG10.8547008547008548No Hit
TAAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCT10.8547008547008548No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAACGTCTAATATT10.8547008547008548No Hit
GCACCTGGATTGGGGTGATACCGGAAGGCGGGCAGGCCCAGCTGGGCCTG10.8547008547008548No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATAC10.8547008547008548No Hit
TAAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACC10.8547008547008548No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATAA10.8547008547008548No Hit
GGTTGTCACTCTGCCGCCAACAGTTTTCGCCTCAAATTCCAATTTGTATA10.8547008547008548No Hit
GATTATATACTATTATTCTAGTTGTTTATAATCAGCGTTATAGGAATATA10.8547008547008548No Hit
AAGGTTAATGTTGTAAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGC10.8547008547008548No Hit
ATTACACTTTCCACTGATATTGAAGGATTTTGGTATCCTCGGGTTGACAT10.8547008547008548No Hit
TAAGGTTAATGATCTATTACCGATCCGAAGCAGCAGCACCT10.8547008547008548No Hit
CTTTTACCCAAAGAACCCATATCCAGTTCTACTCCATCTCCGTTCAATTC10.8547008547008548No Hit
TTAGCGTCATGTTTTACTTACCGATCCGAAGCCAGCAGCACC10.8547008547008548No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAAGCGA10.8547008547008548No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAAA10.8547008547008548No Hit
AGGTGCTGCTGCTGGGATCGGTGGTGGAGGATTTAACCTTAAGCAATATT10.8547008547008548No Hit
GGTGCTGCTGCTTCGGATCGAAGTTAACCTTA10.8547008547008548No Hit
AAAGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGC10.8547008547008548No Hit
TAGTTCTCTCCGTATTGCAAATCAAGACGTTGCCGGATTTTATTTAATTC10.8547008547008548No Hit
GGTGCTGCTGCTCGGAGATACGGTAGTAGAAGATTAACCTTAGCAATACG10.8547008547008548No Hit
GGTAGGGCATGCCGGTGGTTTGGACAAGGCGCAGGTCGTTAAGGAGTCTG10.8547008547008548No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTAAGCAATACGTT10.8547008547008548No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGGTGCTGCT10.8547008547008548No Hit
CACGTCCGGCTTGTCCGTGCTCGGGAGAACCTAAAGAGCCGAACGGTTTG10.8547008547008548No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAA10.8547008547008548No Hit
CGCATGGGAAGCAGAAAATCCGAGCGTTCATGGTGACGCACCGGCATGGC10.8547008547008548No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCGAT10.8547008547008548No Hit
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCA10.8547008547008548No Hit
CTCAGGGAGGAATTGTCTACCCGATACCGGCCGCAGGCTTAAGCCTGATG10.8547008547008548No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACG10.8547008547008548No Hit
GGTGCTGCTGCTTCGGATCGGTAGTGGAGTTTAACCCTAAGCGATGGTAATG10.8547008547008548No Hit
CTATTGATTCTGGGAGAGCTGTTTCTGTGTTAAAACAATCGTTCAGCCTT10.8547008547008548No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACTTTAGCGATCG10.8547008547008548No Hit
GGAAATTAAATGCTTCAAACTTTTTGTCGATAGGGTATGGAGAACATACG10.8547008547008548No Hit
GATGATCTTTCTCGGCTTTCCTTTGAAATACAGAAAGCCAGTTCATGGGC10.8547008547008548No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTT10.8547008547008548No Hit
GGTGCTGCTGCTTCGGATCGGTAGTGGATTAAGTTAGCGATCG10.8547008547008548No Hit
TACCAGGATATCTCTTGTCAGTATTTCCAGTTCCGCAAAGGCTGTTACCT10.8547008547008548No Hit
GGCAGGTATCAGCATCCGCAACTTGGTTGCTGGAGCTGTTTCCATGGAAA10.8547008547008548No Hit
GGCTGCTCAGATAGGTAGTAGAAAGATTAACCTTAGCAATTGGCCATGTG10.8547008547008548No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCGATCGGTG10.8547008547008548No Hit
AGACACT10.8547008547008548No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAAT10.8547008547008548No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAAAAAATAGCTCTTA10.8547008547008548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)