FastQCFastQC Report
Fri 24 Oct 2025
barcode88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebarcode88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length46-4830
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTACAGGAGAGCTCATAAGAGCTCCTCCACCTTTATCATTACCACCCA15.263157894736842No Hit
ATATGCGTCCGCTATGAATGTGTACCCATGCGCTTCATCAAGCATGTCTA15.263157894736842No Hit
AGTTTTGAAAATGCTGCTCCATTTAGCGATTTGGGGAAAAGATATATTTA15.263157894736842No Hit
GGGGGGCCTACGAGGAGGCGCTTGCCTATGCGAGAATGCCGGAGCAGGAC15.263157894736842No Hit
GTCTTTTACTTTTCCCATTATCTGTTATGATGACCGCCACCGAGATACGC15.263157894736842No Hit
TCCACCAGTTGAAGTCGAAATACTTGCAGATATTGGTAGAGGATTCCTCC15.263157894736842No Hit
CGATACCGACGGTGGCAGGCTGGATTATCCAAGCCGGAGGCATGGGAAGC15.263157894736842No Hit
AGCTGGAGGCCGCCGTGGGAGAGGAGGTGGCCCGGTGGTGAAGCTTCTGA15.263157894736842No Hit
TATCAACGGGGAGAATATTACGATTGACGGAGGTATGAGTAAACTAATGA15.263157894736842No Hit
GGTGTTGCTGCTTCGGATCGGTGATGAAGTTAACCTTAAGCAATAAT15.263157894736842No Hit
TTTTCTTCTTGTTCTTGCTGTAGCATTTACTGTGTGGATGCTTCCTTATT15.263157894736842No Hit
AAGCGTAATACGCACGGTATATTAATGCATAAGCATCTAATAGAAAAAGT15.263157894736842No Hit
TCTTCGGAAATCTTCTGAAAGAGAGCGGAAAGACAACTCGACTCGCCAAG15.263157894736842No Hit
GTGCATCGTTCCTTTGATGCACACAACTGTATTATTTGTTATCAGAAGAT15.263157894736842No Hit
GGATGATTTTTCAAGTCGTTAAGTGGATGTTTCTCATTTTCCATGATTTT15.263157894736842No Hit
CCATTTCCAAGAATGAAATATGGCAGTTCTTCATTTTTGCATATCTTCAG15.263157894736842No Hit
GAAAAAGTTAGAACAAGAAAAAATCTTGTAGTTGATACAGTAGCACAAGT15.263157894736842No Hit
CGCGGCTCTCCCTTGGTATTGAAATCTGTGAGTCCGAGGAATGGAATCAT15.263157894736842No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAAT15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)