FastQCFastQC Report
Thu 30 Oct 2025
barcode09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebarcode09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3281
Sequences flagged as poor quality0
Sequence length19-8769
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTTAAAACCAAGACTCGCTGTGCCTAGTTCAGCACCTAAGGTTAAAA1384.20603474550442No Hit
AAGGTTAAAACCAAGACTCGCTGTGCCTAGTTCAGCACCT902.7430661383724475No Hit
AAGGTTAAAACCAAGACTCGCTGTGCCTAGTTCAGCACCA220.6705272782688205No Hit
AAGGTTAAAACCAAGACTCGCTGTGCCTAGTTCAGCACCTGGTGCTGAAC210.6400487656202377No Hit
GGTGCTGAACTAGGCACAGCGAGTCTTGGTTTTAACCTTAGGTGCTGAAC40.12191405059433098No Hit
GGTGCTGAACTAGGCACAGCGAGTCTTGGTTTTAACCTTAGCAATACGT40.12191405059433098No Hit
GGTGCTGAACTAGGCACAGCGAGTCTTGGTTTTAACCTTAGCTAAGGTTA40.12191405059433098No Hit
AAGGTTAAAACCAAGACTCGCTGTGCCTAGTTCAGCACCAGGTGCTGAAC40.12191405059433098No Hit
GGTGCTGAACTAGGCACAGCGAGTCTTGGTTTTAACCTTA40.12191405059433098No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)