FastQCFastQC Report
Thu 30 Oct 2025
barcode01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebarcode01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2286
Sequences flagged as poor quality0
Sequence length27-2796
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCTAAGGTTAACA2189.53630796150481No Hit
AAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT1215.293088363954506No Hit
AAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCTGGTGCTGAAG401.7497812773403325No Hit
AAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCA381.6622922134733158No Hit
GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTA90.39370078740157477No Hit
AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTT70.30621172353455817No Hit
GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGGTGCTGAAG50.21872265966754156No Hit
GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCTAAGGTTA50.21872265966754156No Hit
AAGGTTAACACAAAGACACCGACAACTTTCTTCAGCA50.21872265966754156No Hit
AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGGTGCTGAA40.17497812773403326No Hit
AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAATCGTA30.13123359580052493No Hit
AAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCTGGTGCTGAAGAAAGTTGTCGGTGTCTT30.13123359580052493No Hit
AAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC30.13123359580052493No Hit
AAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCAGGTGCTGAAG30.13123359580052493No Hit
AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCTAAGGTT30.13123359580052493No Hit
AAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCTGGTGCTGAAGAAAGTTGTC30.13123359580052493No Hit
GTGATTTCGTCATTTTTCAAGTCGTCAAGTGGATGTTTCTCATTTTCCAT30.13123359580052493No Hit
AAGGTTAACACAAAGACACCGACAACTTTCTCAGCACCTAAGGTTAACAC30.13123359580052493No Hit
TGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGAC30.13123359580052493No Hit
AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAATACG30.13123359580052493No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)