FastQCFastQC Report
Thu 30 Oct 2025
barcode88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebarcode88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77
Sequences flagged as poor quality0
Sequence length14-2909
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCT45.194805194805195No Hit
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCTAAGGTTAATC33.896103896103896No Hit
AAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCA22.5974025974025974No Hit
GGGCGACGTCGAAGCAGTATGTCGAGATGGTCGCCAGGGATGAGGGCTGG22.5974025974025974No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTA11.2987012987012987No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACATG11.2987012987012987No Hit
CCAGTGCCCTTGCCCTGTTGCCTGTCCCTTCCCCTTCTCCCTGCATCAGG11.2987012987012987No Hit
GTTTCTGAGCCTTTCCGTATCCATGACGAGCTTGACCCTGATCGTCGCGCGTGCAGAACGGCATA11.2987012987012987No Hit
GGTGCTGCTGCTTCGGATCGGTAGATGAAGATTAACCTTAGCAATCGTA11.2987012987012987No Hit
CGTACGATTTCGACGGAGGAGGTCGAATCGCTGCTCGGGCAGCTCTGTGA11.2987012987012987No Hit
TGTGATGCCTGACTAGCCAGAATGTGAAGTCTCACTCTTGCTGTACAGGA11.2987012987012987No Hit
TAAGGTTAATCTTCTACTACCGATGCCGAAGCAGCAGCACC11.2987012987012987No Hit
TCAGTCGCCTTGCTTTCCTTGTATGGCGAAGGCCCGATCACATAGTCACG11.2987012987012987No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGA11.2987012987012987No Hit
TAAGGTTAATCTTCTACTACCGATCCGAAGCAGCAGCACCTCGACCGCTG11.2987012987012987No Hit
AGTTCATAGACCGAACCGCAGCAGCTCATTTTCACATAGTAACGCTTTCC11.2987012987012987No Hit
ACAGTTTGCTCTTTTCAGACATTGCAGGGCAGGGATATCCCGTGTGTTTA11.2987012987012987No Hit
TACTCTCCATGAAGTACCATTGGTGCAAAATACTTCCGTGAAGTACTTAA11.2987012987012987No Hit
CCTCTATATATCATGGAAAAACGTTTCGCAGTAGAAGCTGATTGAATCGC11.2987012987012987No Hit
ATCGTATCTCAACAGAATCGTACCGTTGTGCACTTGATGTACGTCATGTC11.2987012987012987No Hit
GCATTTGCACTAGACGAAATCGAGGATCTTGCCGAAGCCCTTGATGCCGC11.2987012987012987No Hit
CTGGGTGGCCAGCCAGCAATGTCATGGTCTGAATTTGGCTACATGGTGAA11.2987012987012987No Hit
GGTGCTGCTGCTTC11.2987012987012987No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGATTAACCTTAGCAATAAT11.2987012987012987No Hit
GGTCCCTCCAGCATGCTCAGACCATGCTGAGCCGGCCGCATTGAGAGTCG11.2987012987012987No Hit
GGTGCTGCTGCTTCGATGGTAGTAGAAGATTAACCTTAGCAATCTTCGT11.2987012987012987No Hit
AATGAGAAACATCCACTTGACGACTTGAAAAATGACGAAATCACTAAAAA11.2987012987012987No Hit
AAGGTTAATCTTCTACTACCGATCC11.2987012987012987No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCTAAGGTTA11.2987012987012987No Hit
TATCCGACATTGATACCGACGTCATACCGCGCGGCCTGAGCGAGGAGGTG11.2987012987012987No Hit
ATGGAAAATGAGAAACATCCACTTGACGACTTGAAAAATGACAAAATCAC11.2987012987012987No Hit
AAACTTACAAATTTATGCTACTTAAAAACATATGAACTTAAAGCACTTGA11.2987012987012987No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGGTGCTGCTGCTTCGGATCGG11.2987012987012987No Hit
AGATGGAACATTAGTGAGTTTTGAAACTCATAAAGTACTGCAATCTTCTG11.2987012987012987No Hit
CAAAGTGTGGACACTTTACCCCTTCCTAGAAATGGGAAAAAAACACCCTG11.2987012987012987No Hit
ATCAAGGGCGATCGCGTTGAAGTCCGGCAGGATCCGGGGGGCTACCAGTA11.2987012987012987No Hit
ACCTTTCGCAAGACCGGTTATCCGGAGTCTTCTGACTACTTAAAAGGGCT11.2987012987012987No Hit
AGAGTGGAAAAAGAGCAATTTATATTCCATCTGTCACCTCAAAGAAATTC11.2987012987012987No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACATT11.2987012987012987No Hit
ATATGTGCAAAATCAATGACGCAATGCGCTCGATATGCGCTGCAACGTCT11.2987012987012987No Hit
ACGCGACGCCGCATCACGGTTCCAGTACCAGCTGTCGGTCAGCACGAAGC11.2987012987012987No Hit
CTCAGAAACATCATCTTCTCCTATGTGAGGCTCCTTGGAGTGTCTGCAAA11.2987012987012987No Hit
GGTCGCGTGCCCCTGCATACGCTGCGTGCCGACATCGACTACGCAACTTC11.2987012987012987No Hit
CGTATTTATTTAGACTATAATAGACAGAAAGAAGGAAATTGTAAATGAAG11.2987012987012987No Hit
TGCAGCCCGCCGCGGCGCTGATGCTGCACGCGGGCATCGATCTGCTGCCGCCGTGGGCGCAACGCATGCTA11.2987012987012987No Hit
ACAAATTCCATCGAACATCGCAGATTTCGCGTGAAATCTCCGTCGGGCAT11.2987012987012987No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATCGT11.2987012987012987No Hit
GATCCAGCGTGCCGATGGCGCGATTAGCGGCTGTACGAACCTCGATGGCT11.2987012987012987No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACGTGAAC11.2987012987012987No Hit
ATGAGAACGTTCCCCGTATGTTAAAGGACGGCGTTCATGCTGTGATCAGA11.2987012987012987No Hit
GGTGTATGGAGCCAGTATTGTGCTTCATAATCTGTTCCCTTCTACTCAGG11.2987012987012987No Hit
GAGAAGATCTGCTCTTACGCGCTGACGACGTCTCCTTTGATGGTTGCCATATCGAACTCCTGTCGATCGGAGCA11.2987012987012987No Hit
GTAACACTCATCACCTGTCCGGCCTTGCTATACGAATATGTTAAATAGGT11.2987012987012987No Hit
AGCAGTGGATGGGCAAGGGCTTGTACCTCGGCACGCTGATGGTGGGCCTC11.2987012987012987No Hit
CCACCGGATCACCATACTTTTGCTCAAAGCGGATCCGGTAACGTTCAGGT11.2987012987012987No Hit
AAGGTTAATCTTATTACCCGATCCGAAGCAGCAGCACCT11.2987012987012987No Hit
CATCACGCCCGGCGAGGCGCAGGGCGCGGGTGGCATCCCGCGCCCTGCGCATCACACAGAGACGTCCA11.2987012987012987No Hit
GATGATCGTGATGTATAGGCGGTTCTTCCGGAGGGGACGCGGGTGTCGCA11.2987012987012987No Hit
GAGCTGCGCGATGATCCACTTCGAACAGCTGAACCTGACCGATGCCGATT11.2987012987012987No Hit
GCCGCTTTCCTCGGCGACGGGGATGAAGCGCGCCGGCGAAATGAAGCCGT11.2987012987012987No Hit
CGCATGGCGCGCCGCGACTTCAGGCATTTGCGGCCGACGATCGTTTTTTC11.2987012987012987No Hit
AAGTGGAAGGATCCCCTGAGCCCAGGAGGTCAAGGCTACAGTGAGCTCGA11.2987012987012987No Hit
ATTTAATGCTGCTTTTACAACCATAACAGTCTTGCTGCAAACGTTGCTGG11.2987012987012987No Hit
ATGCCTGTATTATTTTTTTTTGATCACTTGATTCGTAGTCCTTTGTTAAG11.2987012987012987No Hit
TCAGCATGGGACGCGGCCACGACGAGGCCCACGCGCCAA11.2987012987012987No Hit
AGCAATTCAACTGGTTCGTGAATTATGCCAATACGTTCGGTAAGCATGAT11.2987012987012987No Hit
GGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCTAAGGTT11.2987012987012987No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCAATACGTAAAA11.2987012987012987No Hit
GTTTGTTTGCTGGCATCGAAGTTTAAACAGGTTTGAAAACATTTATCTCT11.2987012987012987No Hit
AGGTGCTGCTGCTTCGGATCGGTAGTAGAAGATTAACCTTAGCGATG11.2987012987012987No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)