FastQCFastQC Report
Thu 30 Oct 2025
barcode72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebarcode72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533
Sequences flagged as poor quality0
Sequence length33-7053
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTTAATAGCTGACTGTCTTCCATACCGACCAGCACCTAAGGTTAATA60.3913894324853229No Hit
AAGGTTAATAGCTGACTGTCTTCCATACCGACCAGCACCT50.32615786040443573No Hit
AGGTGCTGGTCGGTATGGAAGACAGTCAGCTATTAACCTTAGCAATACAT30.19569471624266144No Hit
CATAACTGCTTGTAACCCCAGCTCCAAGGACCCAAGGCTTCTGTCCTCCA20.1304631441617743No Hit
GTCATGAGACCCGGATTCAGGCGCCATCCGGCTGCTTCAATCCCCCTTAA20.1304631441617743No Hit
CCTCTCCGTTCACTGTACATCCGCTCTTCATTTCACCTGTTGTATAACAT20.1304631441617743No Hit
TAAATGCTGGATAGTTTGTTTCTATTATAGAAACTTGTTAGGTGGCTTAT20.1304631441617743No Hit
TATCATACTTTTTCATGGTTTCCAGAAGGATTTCATGCAGCTGGGAAAAG20.1304631441617743No Hit
GCAGAGAGTGCTCTGGACAGCTGGGACTCAGGACAGAGTTGATCTCCCAG20.1304631441617743No Hit
TGCCCTAGAAAAAACAAGAAGGTAATCCCTCAACAAACCTAAAAGAAGAC20.1304631441617743No Hit
TACTCATAACCATAGCCGCTTCTTTCTCCCTTTTCATTGACGGTATTATA20.1304631441617743No Hit
GGGAGCGTGTGGGAGACTTTGGGATAGCATTGGAAATGTAATTGAGGAAA20.1304631441617743No Hit
ACTGTTGGAATCTGGTCATATTTATGATCTGATTTCCTCATATCAAGTAA20.1304631441617743No Hit
GAAACTAGAGGGGAAACCCCCACTCATATCCCAAGTCGTGCACCCCAAAG20.1304631441617743No Hit
CACCATCCGGACATCATAGGTGAGATTTTGCTATCCTGGGTTCAGTTACT20.1304631441617743No Hit
CATCCAGCACCACCGCCATCGGATTGACTGCAGCTTAGAATCTCTCTGAG20.1304631441617743No Hit
ATGTGGGAACACATCCGCTTTATCTGTCAGAAGTAATGCTTCCCGGATAT20.1304631441617743No Hit
CCCAGGTGTGCCAAACACCCCCACAAATCAGAAGATTGTCAGCATTTGCA20.1304631441617743No Hit
TAATCTGTTCTTTATTCAGCATATGGAAAACAATAATCTCATCAATACGG20.1304631441617743No Hit
GAAAATCACAAATAAAAGTTACATATATTAATACAGATGTGACTTTCTAA20.1304631441617743No Hit
GAAAATGAGAAACATCCACTTGACGACTTGAAAAATGACGAAATCACTAA20.1304631441617743No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)