FastQCFastQC Report
Thu 30 Oct 2025
barcode56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebarcode56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153
Sequences flagged as poor quality0
Sequence length27-7360
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTTAATCACTACTCAACAGGTGGCATGAACAGCACCT544.683434518647007No Hit
AAGGTTAATCACTACTCAACAGGTGGCATGAACAGCACCTAAGGTTAATC322.7753686036426712No Hit
AAGGTTAATCACTACTCAACAGGTGGCATGAACAGCACCA40.3469210754553339No Hit
AAGGTTAATCACTACTCAACAGGTGGCATGAACAGCACCTGGTGCTGTTC30.26019080659150046No Hit
TCCTTGATTTCCAAATTTGTAAAATCCGTGGAAACATCAAAGTCCTGTCC20.17346053772766695No Hit
AAGGTTAATCACTACTCAACAGGTGGCATGAACAGCACCTGAATGAGAAG20.17346053772766695No Hit
GAATATCCGCAAGAAGATTTCCTATTCCCCCAGCATCACCGATGAAATCA20.17346053772766695No Hit
AAAGGTCAGCCAGCAGCAGGGAGGGCAAGCCAGACATGGAAATATAACAG20.17346053772766695No Hit
CATGTCCTCTTAGAGTTGATATGAATGATTCCATTAAGTTCAATTCAGAT20.17346053772766695No Hit
AAGGTTAATCACTACTAGACAGGTGGCATGAACAGCACCT20.17346053772766695No Hit
AAGGTTAATCACTACTCAACAGGTGGCATGAACA20.17346053772766695No Hit
TCAGTACCTCGTAAATCACGCAACAGATGACCGTACACAGATCCGTCCAC20.17346053772766695No Hit
ACGTGAAATATGGCGAGGAATACTGAAAAAGATGGAAAATTAAAAATGTC20.17346053772766695No Hit
GGTGCTGTTCATGCCACCTGTTGAGTAGTGATTAACCTTA20.17346053772766695No Hit
GGTGCTGTTCATGCCACCTGTTGAGTAGTGATTAACCTTAGGTGCTGTTC20.17346053772766695No Hit
CAAAAAAGTCAAATAATAAGGAAGGCCCAAGTGAAGATGGTTCAATCTTT20.17346053772766695No Hit
CAGGAACTATCTTCGTGGACTATGAAAATAAAGGAGATTTCAGTGACTGC20.17346053772766695No Hit
GGGACAGGCTATGCAGTGGGATCTCCTTGATGCACATCTGCAAGTGAGTT20.17346053772766695No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)